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. 2020 Dec 15;12(12):7855–7869.

Figure 2.

Figure 2

Circ_USP36 knockdown inhibited proliferation, migration and invasion of ox-LDL-induced HUVSMCs. HUVSMCs treated with 25 μg/mL ox-LDL were transfected with blank, si-NC or si-circ. A. QRT-PCR assay for circ_USP36 expression in ox-LDL-induced HUVSMCs after transfection. Unpaired t-test was used to compare data with normal distribution and equal variance between two groups. B. CCK-8 assay for cell viability of ox-LDL-induced HUVSMCs after transfection. C, D. Transwell assay for cell migration and invasion of ox-LDL-induced HUVSMCs after transfection. E. Flow cytometry for cell apoptosis of ox-LDL-induced HUVSMCs after transfection. F. Western blot assay for levels of N-cad, E-cad, Bax, MMP9 and PCNA proteins in ox-LDL-induced HUVSMCs after transfection. B-F. One-way ANOVA was applied for comparison of data among multiple groups, followed by Tukey’s post hoc test. *P < 0.05.