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. 2020 Dec 15;12(12):7855–7869.

Figure 6.

Figure 6

MiR-182-5p could repress the proliferation and metastasis of ox-LDL-induced HUVSMCs by targeting KLF5. (A and B) QRT-PCR and western blot assays for KLF5 mRNA (A) and protein (B) expression in HUVSMCs transfected with vector or oe-KLF5. Unpaired t-test was used to compare data with normal distribution and equal variance between two groups. (C-G) Ox-LDL-induced HUVSMCs were transfected with blank, mimic NC, miR-182-5p mimic, miR-182-5p mimic + vector or miR-182-5p mimic + oe-KLF5. One-way ANOVA was applied for comparison of data among multiple groups, followed by Tukey’s post hoc test. (C) CCK-8 assay for cell viability of ox-LDL-induced HUVSMCs after transfection. (D and E) Transwell assay for cell migration and invasion of ox-LDL-induced HUVSMCs after transfection. (F) Flow cytometry for cell apoptosis of ox-LDL-induced HUVSMCs after transfection. (G) Western blot assay for levels of N-cad, E-cad, Bax, MMP9 and PCNA proteins in ox-LDL-induced HUVSMCs after transfection. *P < 0.05.