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. 2020 Dec 15;12(12):8111–8122.

Figure 2.

Figure 2

Surgery but not anesthesia downregulated SIRT1 expression in the hippocampus. (A) Image of western blot protein bands for SIRT1 and (B) relative expression of SIRT1 mRNA in the hippocampus in each group, with β-actin as an endogenous control. (C) Immunofluorescence analysis detected SIRT1 protein levels in the CA1 and DG region of the hippocampus (scale bar = 50 µm). Data were presented as mean ± SEM. *P < 0.05, **P < 0.01.