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. 2020 Dec 15;12(12):8099–8110.

Figure 2.

Figure 2

Effect of shikonin on osteogenic differentiation in BMSCs. A. BMSCs were cultured with OM (control) and shikonin. All the assays were performed using ALP staining assay after 7 days. B, C. BMSCs were cultured with OM (control), with or without shikonin for 14 days. All the assays were performed and quantified by Alizarin Red S staining. *P < 0.05 and #P < 0.01 vs the control group (n=3). D. ALP activity was tested on BMSCs cultured with OM and shikonin for 7 days. *P < 0.05 and #P < 0.01 vs the control group (n=3). E-G. BMSCs cultured with OM (control), shikonin (50, 150, 250 nM) were harvested on 3 day and 5 day. The mRNA expression levels of ALP, col-1 and OCN were assessed by qRT-PCR and quantified *P < 0.05 and #P < 0.01 vs the control group.