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Shikonin inhibited the differentiation of BMMs indirectly. A. NFATc1 and c-Fos expression in BMMs treated with the indicated concentrations of supernatant for 48 h and quantified. *P < 0.05 and #P < 0.01 vs the control group. B-D. TRAP-positive BMMs treated with indicated concentrations of supernatant followed by the stimulation with M-CSF and RANKL for 5 days. Quantification of TRAP-positive multinuclear cells, area of osteoclasts.
