Figure 3.

Overexpression of KLF4 suppresses the progression of OA in an OA mouse model. A. Safranin O and fast green staining was used to assess the pathological changes of cartilage tissues in an ACLT-induced OA mouse model. B. Immunohistochemistry was presented to detect the expression of KLF4 in an OA mouse model. C. Safranin O and fast green staining was presented to detect the pathological changes of cartilage tissues in an OA mouse model following injection with pcDNA3.1-KLF4. Magnification: 200×. D, E. COL2A1, Aggrecan, MMP3, MMP13 and ADAMTS-4 expression was examined in OA cartilage tissues using RT-qPCR. ***P < 0.001; ****P < 0.0001.