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. 2020 Dec 15;12(12):7737–7761.

Figure 2.

Figure 2

Effect of oleic acid on cell migration in CD36-deficient MHECs and MLECs. A. Effect of oleic acid (OA), linoleic acid (LA), combination of OA+ LA or palmitic acid (PA) (300 µmol/L) on MHEC and MLEC migration compared to control (CTRL) ECs. Representative images of wound healing assays imaged at 0 h and 16 h. White lines were added to show the width of the wound. Histograms show percent wound closure over 16 hours. Data represent mean ± SEM, n = 6, *P < 0.05 vs. CTRL; B. Effect of oleic acid (OA, 300 µmol/L) on siRNA-mediated CD36 knockdown on MHEC and MLEC migration compared to NT siRNA ECs. Representative images of wound healing assays imaged at 0 h and 16 h. White lines were added to show the width of the wound. Histograms show percent wound closure over 16 hours. Data represent mean ± SEM, n = 6, *P < 0.05 vs. NT siRNA; C. Effect of oleic acid (OA, 300 µmol/L) on ASO-mediated CD36 knockdown on MHEC and MLEC migration compared to NT ASO ECs. Representative images of wound healing assays imaged at 0 h and 16 h. White lines were added to show the width of the wound. Scale bars represent 100 μm. Histograms show percent wound closure over 16 hours. Data represent mean ± SEM, n = 6, *P < 0.05 vs. NT ASO. Two-way ANOVA statistical tests were used to determine statistical significance.