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. 2020 Dec 15;12(12):7737–7761.

Figure 4.

Figure 4

Effect of oleic acid on gene expression of endothelial and angiogenic markers in CD36-deficient MHECs. Effect of oleic acid (OA, 300 µmol/L) and siRNA-mediated CD36 knockdown on MHEC endothelial and angiogenic marker mRNA expression: (A) CD36, CD31, eNOS (B) VEGFα, VEGFR2, VEGFβ, VEGFR1 and (C) MMP9, MMP2, TIMP1, TIMP2, TIMP3, TIMP4 assessed by quantitative realtime PCR (qRT-PCR) normalized to 18S mRNA expression. Histograms show fold change in mRNA expression compared to NT siRNA ECs. Data represent mean ± SEM, n = 6, *P < 0.05 vs. NT siRNA, °P < 0.05 vs. NT siRNA + OA, #P < 0.05 vs. CD36 siRNA; (D) MMP9/MMP2 release assessed by colorimetric ELISA assays determined at 450 nm. Histograms show relative units (RU) of MMP release in supernatant samples of MHECs and MLECs treated with BSA (CTRL) or BSA-bound OA (OA) for 16 hours. Data represent mean ± SEM, n = 7, **P < 0.01 vs. MHECs CTRL. Two-way ANOVA statistical tests were used to determine statistical significance.