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. 2020 Dec 15;12(12):7737–7761.

Figure 5.

Figure 5

Effect of oleic acid on gene expression of metabolic markers and protein expression of AMPK in CD36-deficient MHECs. Effect of oleic acid (OA, 300 µmol/L) and siRNA-mediated CD36 knockdown on MHEC metabolic gene mRNA expression: (A) PPARα, PPARδ, PPARγ (B) AMPKα, ATGL, FASN, PLIN2, Cpt1a. Cpt1b, GLUT4, and GLUT1. Histograms show fold change in mRNA expression compared to NT siRNA ECs. Data represent mean ± SEM, n = 5, *P < 0.05 vs. NT siRNA, °P < 0.05 vs. NT siRNA + OA, #P < 0.05 vs. CD36 siRNA; (C) Effect of oleic acid (OA, 300 µmol/L) and insulin (50 units/mL) and siRNA-mediated CD36 knockdown on MHEC intracellular signaling proteins as assessed by western blot. Images of SDS-PAGE gels represent protein expression of phospho-AMPK, AMPK and housekeeping gene α-tubulin. Histograms show fold change in protein expression compared to NT siRNA ECs. Data represent mean ± SEM, n = 3, *P < 0.05 vs. NT siRNA. Two-way ANOVA statistical tests were used to determine statistical significance.