Figure 4.

GREB1 activates Akt through intracellular mechanisms. (A) Conditioned media from MCF7 cells transduced with GFP or GREB1 adenovirus was added to untransduced cells. Cell lysates from transduced cells (GFP or GREB1) and untransduced cells cultured in conditioned media (GFP CM or GREB1 CM) were harvested 24 h later. Lysates were analyzed by immunoblot for indicated proteins. (B) MCF7 cells were transduced with adenovirus expressing GFP or GREB1. Transduced cells were then cultured with untransduced cells at a 1:1 ratio for 24 h. Cells were harvested and sorted for GFP. Cell lysates were analyzed via immunoblot for expression of the indicated proteins.