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. 2020 Jul 25;41(12):1648–1659. doi: 10.1093/carcin/bgaa047

Figure 2.

Figure 2.

Treatment with low-dose PAH mixture does not affect viability, proliferation or the AhR toxicant response pathway in normal mammary or ER− breast cancer cells. For (A) hTERT-HME1 normal human mammary, (B) MDA-MB231 triple negative and (C) SUM149 triple negative breast cancer cells: Viability counts following low-dose PAH mixture or 1 µM BaP single chemical treatment for 24 h. BaP, benzo(a)pyrene; Ctrl, 5 µM chlorothalonil cytotoxic chemical control. #P < 0.0001. Proliferation by MTT assay following low-dose PAH mixture or 1 µM BaP single chemical treatment for 24 h. Cropped immunoblot of the AhR with densitometry values normalized to GAPDH and compared with untreated. AhR (100 kD), aryl hydrocarbon receptor; GAPDH (36 kD), glyceraldehyde 3-phosphate dehydrogenase. CYP1A1/CYP1B1 enzyme activity by ethoxyresorufin-O-deethylase assay analysis in following 24 h PAH mixture treatment or treatment with 1 µM BaP single chemical.