Table 2.
Digitally quantified CD3, CD4, CD8, CD20 and CD45RO density (cells/mm2) and a proposed TNM-Immune cell score (a combination of CD8 TIL density and pStage) as predictors of disease-specific survival in resected NSCLC patients and in subgroups with squamous cell carcinoma and adenocarcinoma (univariable analyses, log-rank test, n = 553, 307 and 239, respectively)
| All patients | LUSC | LUAD | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N (%) | 5 years | Median | HR (95% CI) | P | N (%) | 5 years | Median | HR (95% CI) | P | N (%) | 5 years | Median | HR (95% CI) | P | |
| CD3 | <0.001 | 0.002 | 0.071 | ||||||||||||
| ≤1000 | 384 (69) | 53 | 83 | 1.000 | 221 (72) | 59 | NA | 1.000 | 160 (67) | 47 | 57 | 1.000 | |||
| >1000 | 140 (25) | 72 | 235 | 0.53 (0.4–0.71) | 72 (23) | 83 | 235 | 0.44 (0.29–0.67) | 64 (27) | 60 | NA | 0.66 (0.44–1) | |||
| Missing | 29 (5) | 14 (5) | 15 (6) | ||||||||||||
| CD4 | 0.007 | 0.009 | 0.206 | ||||||||||||
| ≤550 | 319 (58) | 54 | 98 | 1.000 | 191 (62) | 58 | 235 | 1.000 | 124 (52) | 49 | 57 | 1.000 | |||
| >550 | 207 (37) | 66 | NA | 0.68 (0.51–0.89) | 104 (34) | 78 | NA | 0.56 (0.37–0.83) | 101 (42) | 55 | NA | 0.78 (0.53–1.14) | |||
| Missing | 27 (5) | 12 (4) | 14 (6) | ||||||||||||
| CD8 | <0.001 | <0.001 | 0.04 | ||||||||||||
| ≤500 | 242 (44) | 46 | 47 | 1.000 | 136 (44) | 47 | 41 | 1.000 | 105 (44) | 45 | 51 | 1.000 | |||
| >500 | 282 (51) | 70 | 235 | 0.48 (0.36–0.63) | 155 (50) | 81 | 235 | 0.35 (0.24–0.53) | 121 (51) | 56 | NA | 0.68 (0.46–0.99) | |||
| Missing | 29 (5) | 16 (5) | 13 (5) | ||||||||||||
| CD20 | <0.001 | 0.046 | 0.003 | ||||||||||||
| ≤400 | 431(78) | 55 | 98 | 1.000 | 245 (80) | 62 | 235 | 1.000 | 180 (75) | 45 | 54 | 1.000 | |||
| >400 | 100 (18) | 76 | NA | 0.48 (0.34–0.66) | 52 (17) | 77 | NA | 0.55 (0.34–0.89) | 47 (20) | 74 | NA | 0.41 (0.26–0.64) | |||
| Missing | 22 (4) | 10 (3) | 12 (5) | ||||||||||||
| CD45RO | <0.001 | 0.002 | 0.173 | ||||||||||||
| ≤250 | 339 (61) | 54 | 83 | 1.000 | 188 (61) | 58 | 127 | 1.000 | 147 (62) | 49 | 57 | 1.000 | |||
| >250 | 187 (34) | 67 | NA | 0.6 (0.46–0.79) | 106 (35) | 77 | NA | 0.51(0.34–0.75) | 78 (33) | 54 | NA | 0.76 (0.51–1.11) | |||
| Missing | 27 (5) | 13 (4) | 14 (6) | ||||||||||||
| TNM-I | <0.001 | <0.001 | <0.001 | ||||||||||||
| Fav | 121 (22) | 83 | 235 | 1.000 | 62 (20) | 89 | 235 | 1.000 | 55 (23) | 76 | NA | 1.000 | |||
| Int-Fav | 166 (30) | 69 | 190 | 2.03 (1.44–2.84) | 95 (31) | 76 | NA | 2.24 (1.36–3.68) | 70 (29) | 61 | 190 | 1.78 (1.11–2.85) | |||
| Int-Poor | 155 (28) | 47 | 57 | 3.39 (2.35–4.89) | 93 (30) | 58 | NA | 3.52 (2.08–5.96) | 61 (26) | 33 | 44 | 3.38 (2.01–5.69) | |||
| Poor | 82 (15) | 21 | 16 | 7.79 (4.7–12.92) | 41 (13) | 18 | 14 | 12.57 (5.68–27.82) | 40 (17) | 21 | 36 | 4.73 (2.46–9.1) | |||
| Missing | 29 (5) | 16 (5) | 13 (5) | ||||||||||||
P-values below 0.05 are highlighted in bold. CD, cluster of differentiation; NA, not applicable.