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. 2021 Jan 7;12:38. doi: 10.1186/s13287-020-02084-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — a Alizarin Red staining for calcium nodule formation in the control and Botox-injected group cultured in either basal or osteogenic induction medium (OIM) showing the decreased calcium nodules formation in the Botox group. b Quantitation of Alizarin Red by read at 595 nm of destaining solution by 96-well plate reader and normalised to basal-medium control group (n = 3; mean ± SD; *p < 0.05, **p < 0.005, *** p < 0.001). c mRNA expressions of osteogenic differentiation markers (ALP, BMP2 and RUNX2) in the control and Botox group. Individual mRNA expression levels were first normalised to internal control (36B4) and then normalised against mRNA expression level from the control group. (n = 3; mean ± SD; **p < 0.005, ***p < 0.001)

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