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. 2021 Jan 7;12:45. doi: 10.1186/s13287-020-02112-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Cell proliferation and colony-forming of BMSCs from 2M, 12M-VEH and 12M-H-Desferal group rats. a Schema of short-term Desferal® administration of the ex vivo study. b CCK-8 assay of cell proliferation. The optical density (OD) value was measured at 450 nm absorbance. In BMSCs from 12M-H-Desferal® group, the proliferation rate (OD value) significantly increased compared with those from 12M-VEH group. While the OD values of 12M-VEH group are lower than those from 2M group at day 1–3. The data were drawn from three independent experiments and the results were expressed as mean ± SD. *p < 0.05, ***p < 0.001. c Colony-forming cell assay. Decreased cell colonies in BMSCs from the 12M-VEH group compared with that from the 2M group. While cell colonies in BMSCs from the 12M-H-Desferal® group increased significantly compared with that from the 12M-VEH group