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. 2021 Jan 7;12:45. doi: 10.1186/s13287-020-02112-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Osteogenic and adipogenic differentiation of BMSCs from rats with and without Desferal® treatment. a Osteogenic differentiation was determined by ALP staining at day 7 and Alizarin Red S staining at day 18 after osteogenic induction. Scanned images from a 24-well plate. b Higher magnification images of ALP and Alizarin Red S staining. Bar=500 μm. c ALP activities of BMSCs after osteogenic differentiation for 7 days and quantitative analysis of Alizarin Red S staining at day 18. *p < 0.05, **p < 0.01. d Oil red-O staining for adipocytes after adipogenic differentiation for 21 days. Top bar = 500 μm, bottom bar = 100 μm