Fig. 5.

Exosomal miR-27b from MSCs suppresses the JMJD3/NF-κB/p65 axis to inhibit the development of CLP-induced sepsis. a The expression of JMJD3 and H3K27me3 in mice subjected to sham operation or CLP in response to MSC-miR-27b-mimic-EXO analyzed by Western blot. *p < 0.05 vs. mice subjected to CLP; #p < 0.05 vs. mice subjected to sham operation. b Results of a 7-day survival in mice. n = 10 for mice following each treatment. c The production level of TNF-α, IL-1β, IL-10, and IL-6 in mouse serum examined by ELISA. d Bacterial colonies in peritoneal fluid and serum of mice. e Detection of the liver and kidney function in mice. *p < 0.05 vs. mice subjected to CLP. n = 10 for mice following each treatment. Cellular experiments were repeated 3 times independently. Quantitative data were presented as mean ± standard deviation. Comparisons among multiple groups were analyzed by one-way ANOVA with Tukey’s post hoc test. p < 0.05 indicated significant difference