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. 2021 Jan 8;18:3. doi: 10.1186/s12979-021-00214-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Age- and sex-dependent changes in cytokine/chemokine concentrations following whole blood stimulation. Whole blood samples were collected from young and aged male and female mice. Samples were either unstimulated or stimulated with 10 ng/mL LPS for 24 hrs. a Plots represent multiplex pro-inflammatory cytokine analysis of young and aged males following whole blood stimulation. b Plots represent multiplex pro-inflammatory cytokine analysis of young and aged females following whole blood stimulation. Young males (n = 3–8, 2–3 months); young females (n = 5, 2–3 months); aged males (n = 5, 18–19 months); aged females (n = 5, 18–19 months). Data were analyzed by 2-way ANOVA with Fisher’s LSD post hoc test. Data represent mean ± SEM. Age: #p < 0.05, ##p < 0.001, ###p < 0.001; Stimulation: *p < 0.05, **p < 0.01, ***p < 0.001