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. 2021 Jan 8;22:25. doi: 10.1186/s13059-020-02208-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Evolution of the rhodopsin regulatory networks in O. niloticus, A. burtoni and M. zebra. a On the left, GATA2A motif prediction in reverse orientated O. niloticus and A. burtoni rhodopsin gene promoter (red box) and substitution demarcated in M. zebra rhodopsin gene promoter (red arrow). On the right, GATA2A partial protein alignment showing DNA-binding domain (DBD) annotation in human, mouse, O. niloticus, A. burtoni and M. zebra. b EMSA validation of GATA2A DBD binding to O. niloticus and A. burtoni rhodopsin gene promoter. Table denotes combinations of DNA probe and expressed DBD in EMSA reactions that include negative controls (lanes 1 to 5); O. niloticus (lane 6), A. burtoni (lane 7) and M. zebra (lane 8) protein: DNA-binding assays. GATA2A:rho complex formed in O. niloticus (lane 6) and A. burtoni (lane 7) as confirmed by band shift (red box) and no complex formed in M. zebra (lane 8)