Figure 3.

4-OI inhibited oxidative stress in LPS-induced acute lung injury mice. Treatment of mice with 4-OI at doses of 25 mg/kg was administered intraperitoneally, and the control group received an equivalent volume of vehicle [(2-hydroxypropyl)-β-cyclodextrin in phosphate-buffered saline (PBS)] 2 h before saline or LPS injection (5 mg/kg, intratracheal). In total, 12 h later, ROS generation (A) in the BALF, MDA content (B), SOD activity (C) and GSH content (D) in lung tissue were determined (n=5). Data are expressed as the mean ± SEM. ^*P<0.05. 4-OI, 4-octyl itaconate; LPS, lipopolysaccharides; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase; GSH, glutathione.