Fig. 5.

ACTN1 competitively interacts with MOB1 to regulate Hippo signaling activity. a Co-immunoprecipitation analysis of the physical interaction between ACTN1 and MOB1 in the Huh-7 cells. b Co-immunoprecipitation analysis of the interaction between ACTN1 and MOB1 in the MHCC-97H cells. c Co-immunofluorescence analysis of ACTN1 and MOB1 expression in the Huh-7 cells. Scale bar: 10 μm. d Western blotting analysis of phospho-LATS1, LATS1, phospho-YAP and YAP in lenti-ACTN1 and lenti-vector Huh-7 cells. Statistical analyses of densitometry were shown at right. The experiments were repeated three times. e Western blotting analysis of effect of MOB1 knockdown on the activity of Hippo signaling pathway. Statistical analyses of densitometry were shown at right. The experiments were repeated three times. f Real-time qPCR analysis of the mRNA levels of CTGF, ANKRD1 and CYR61 in lenti-ACTN1 and lenti-vector Huh-7 cells. The experiments were repeated three times. g CCK8 experiment of the cell viability of lenti-ACTN1 and lenti-vector Huh-7 or LM3 cells upon MOB1 knockdown in the presence or absence of 50 nM Verteporfin or 50 nM Super-TDU (1–31) (inhibitors of YAP-TEAD complex). The cell viability of Huh-7 and LM3 cells was analyzed after 24, 48 and 72 h. The experiments were repeated three times. *P < 0.05; **P <  0.01