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. 2021 Jan 7;21:28. doi: 10.1186/s12879-020-05729-6

Table 1.

PCR primers sequences

Gene name Primer sequence Annealing temperature Product length (bp)
IL - 2 FP: 5′-AACCTCAACTCCTGCCACAA-3′ 60 °C 197
RP: 5′-GCATCCTGGTGAGTTTGGGA-3′
IL - 4 FP: 5′-CACAACTGAGAAGGAAACCTTCTG-3′ 60 °C 253
RP: 5′-CTCTCTCATGATCGTCTTTAGCCTTTC-3′
IL - 10 FP: 5′-AGAACCT GAAGACCCTCAGGC-3’ 60 °C 78
RP: 5′-CCACGGCCTTGCTCTTGTT-3’
IL - 13 FP: 5′-GCTCCTCAATCCTCTCCTGTT-3’ 58 °C 485
RP: 5′-GCAACTTCAATAGTCAGGTCC-3’
GAPDH FP: 5′-GAAGGTGAAGGTCGGAGTC-3’ 60 °C 226
RP: 5′-GAAGATGGTGATGGGATTTC-3’