Fig. 7.

Western-blotting analysis of HA2 protein from mutant viruses. Lysates (amount of protein was 50 μg) of chicken embryo fibroblasts (CEF) infected with mutant viruses at MOI of 0.01 for 12 h–15 h were incubated with a primary antibody against H5 14th peptide and monoclonal antibody (mAb) against β-actin. Bands were visualized by a chemiluminescence imaging analysis system after incubation with horse radish peroxidase (HRP)-labeled secondary antibodies. S-Lo: H5 IAV control strain; S-Y-G: Tyrosine (Y) in 14–4 peptide of H5 IAV was mutated by Glycine (G); S-H-G: Histidine (H) in 14–4 peptide of H5 IAV was mutated by G; S-K-G: Lysine (K) in 14–4 peptide of H5 IAV was mutated by G; S-D-G: Asparticacid (D) in 14–4 peptide of H5 IAV was mutated by G