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. 2021 Jan 7;12:22. doi: 10.1186/s13287-020-02059-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Identify NSCs and the effect of CGP on cell viability of NSCs. a–c The confocal microscopic images of neurosphere that stained with nestin (green) and counterstained with DAPI (blue) as the nuclear marker (scale bar 100 μm). d–f The confocal microscopic images of single neural cells stained with nestin (green) and counterstained with DAPI (blue) as the nuclear marker (scale bar 100 μm). g The histogram showing the cell viability values detected by the CCK8 assay for each group. **P < 0.01 compared with the vehicle group; ^#P < 0.05, ^##P < 0.01 compared with the 100 nM CGP group (the data are expressed as the mean ± SD, n = 6 samples per condition; unpaired Student’s t test with Kolmogorov–Smirnov test)