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. 2021 Jan 7;21:15. doi: 10.1186/s12903-020-01366-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of stem cells from four types of dental tissue. Scale bar = 100 µm. a Cell morphology was observed at passage three by phase contrast microscope. The fibroblast-like dish-adherent growth cell morphologies were observed in all dental stem cells. b All dental stem cells were successfully differentiated into mesenchymal lineages, including adipocytes, and chondrocytes, which were detected by lineage-specific cytochemical staining: Oil red O for adipocytes, and alcian blue for chondrocytes. c The gene expression of lineage specific markers in differentiated adipocytes and chondrocytes in RT-qPCR. Data are represented by the mean ± SD of four independent experiments. Lettered subscripts indicate statistical differences between groups (p < 0.05)