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. 2021 Jan 7;21:15. doi: 10.1186/s12903-020-01366-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Analysis of E2 supplementation in odontoblastic differentiation of DPSCs. The mRNA levels of odontoblast specific markers ALP, DMP-1, and DSPP were highest in odonto/osteoblasts supplemented with E2. Levels were similar to those in cultured pure odontoblasts from dental chamber (Odontoblast). Expression of ER-α was highest in E2 supplemented differentiated cells. Data are represented by the mean ± SD of four independent experiments. Lettered subscripts indicate statistical differences between groups (p < 0.05)