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. 2021 Jan 7;12:44. doi: 10.1186/s13287-020-02107-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Immunophenotype and molecular profile of CD146-selected IFP-MSC spheroids. a Upon seeding in gas-permeable culture plates, CD146POS IFP-MSC spheroids show as expected higher expression levels of CD146 (451 ± 4 AU vs 241 ± 18 AU) and higher expression levels of NG2 (362 ± 178 AU vs 287 ± 22 AU), whereas CD146NEG IFP-MSC spheroids show higher expression levels of CD10 (253 ± 27 AU vs 164 ± 13 AU) and CXCR4 (227 ± 3 AU vs 140 ± 14 AU). b Molecular profiling of CD146POS versus CD146NEG IFP-MSC spheroids revealed that 55 out of 90 genes tested were higher expressed in CD146POS IFP-MSC spheroids with 12 genes being more than twofold higher (MCAM, HGF, ALPL, DLX5, NES, MMP13, CD200, COL1A1, FZD9, PDGFRB, HIC1). Genes tested were grouped in phenotype/function-related cohorts. c A total of 26 genes are shared between Crude and CD146POS spheroids, indicating similarities in their molecular signatures. However, upon TI or TIC induction, most of the immunoregulatory-related genes tested show upregulated expression levels in CD146POS spheroids. All experiments were performed independently (n = 3)