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. 2021 Jan 7;11:9. doi: 10.1186/s13578-020-00525-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Selonsertib reverses JNK-mediated DRP1 mitochondrial translocation and abnormal mitochondrial dynamics. a, b Western blot analysis and grey value assay of p-DRP1 and DRP1 levels in murine whole liver samples (a) and the mitochondrial fraction of liver samples (b) at initial (0 h) and 0.5 h, 1 h, 2 h, and 4 h after LPS/GalN injection. c, d Western blot analysis and grey value assay of p-DRP1 and DRP1 levels in murine whole liver samples (c) and the mitochondrial fraction of liver samples (d) from different groups. e Western blot analysis and grey value assay of the expression levels of mitochondrial dynamics-related proteins in murine liver samples. f–h Anisomycin was used to further confirm the role of JNK in mediating the effects of selonsertib on DRP1 activation and mitochondrial dynamics. Western blot analysis and grey value assay of DRP1 activation and mitochondrial translocation in murine whole liver samples (f) and the mitochondrial fraction of liver samples (g) and the expression levels of mitochondrial dynamics-related proteins in murine liver samples (h). Two from 6 samples were shown in the blots. Data are presented as the mean ± SD (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001; NC normal control, SEL selonsertib (30 mg/kg), AN anisomycin (20 mg/kg)