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. 2021 Jan 7;12:19. doi: 10.1186/s13287-020-02076-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — YAP activation and nuclear translocation contributed to radial shockwave-mediated promotion of self-renewal of SCB-SPCs. a The YAP/TAZ expression level exhibited a consistent tendency with CFU-F under the respective doses of r-ESW stimulation: control group, 1A (1 bar, 300 times), 2B (2 bar, 600 times), and 3D (3 bar, 1200 times) (n = 5, with each 3 technical repeats). b Immunofluorescence of YAP localization and semiqualitative evaluation of YAP distribution in SCB-SPCs among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). c Immunofluorescence of Sox2 expression and semiqualitative evaluation in SCB-SPCs among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). d Immunofluorescence of Nanog expression and semiqualitative evaluation in SCB-SPCs among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). e The relative expression levels of self-renewal-associated genes in SCB-SPCs among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). f The general staining and qualitative analysis of CFU-F among the control, r-ESW, and r-ESW + verteporfin groups (n = 5, with each 3 technical repeats). Scale bars represent 25 μm. *p < 0.05, **p < 0.01, ***p < 0.001