Figure 4. Glucose specific regulation of ketone body utilizing protein activity and cardiac metabolic rates.

A, Catalytic activity of BDH1 in mitochondrial fractions prepared from the hearts of mG4H mice. B, SCOT enzymatic activity was measured in the CoA transferase–enriched fractions from the heart. C, After 2 weeks of transgene induction, hearts from mG4H mice were isolated and perfused ex vivo in presence of D‐[5‐³H]‐glucose for glycolysis (C) and [U‐¹⁴C]‐βOHB for oxidation (D). Values are expressed as means±SEM (n=5–9; sample size range varies dependent on the parameter investigated); *P<0.05; ***P<0.001; vs. Con. BDH1 indicates β‐hydroxybutyrate dehydrogenase; MG4H, transgenic inducible cardiac‐restricted expression of glucose transporter 4; SCOT, succinyl‐CoA:3‐oxoacid CoA transferase.