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. 2020 Jul 30;9(15):e013039. doi: 10.1161/JAHA.119.013039

Figure 5. Protein posttranslational regulation via O‐GlcNAcylation is enhanced by STZ, HFD, and glucose.

Figure 5

Cardiac protein O‐GlcNAcylation was measured in ventricular lysates prepared from 4 weeks STZ‐induced diabetic mice, 12‐week HFD, or Con diet fed mice and mG4H mice after 2 weeks of transgene induction. Fifty micrograms of lysates was resolved and probed using CTD110.6 antibody. A, Upper panel: immunoblot illustrating O‐GlcNAc detection in ventricular tissue of STZ mice compared with vehicle‐treated mice. Lower panel: Densitometric analysis of immunoblots shown in upper panel. B, Upper panel: immunoblot illustrating O‐GlcNAc detection in ventricular tissue of HFD mice compared with vehicle‐treated mice. Lower panel: Densitometric analysis of immunoblots shown in upper panel. (C) Upper panel: immunoblot illustrating O‐GlcNAc detection in ventricular tissue of mG4H mice compared with vehicle‐treated mice. Lower panel: Densitometric analysis of immunoblots shown in upper panel. *P<0.05. Con indicates Control; HFD, high‐fat diet; MG4H, transgenic inducible cardiac‐restricted expression of glucose transporter 4; STZ, streptozotocin; Veh, Vehicle.