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. 2021 Jan 7;9:10. doi: 10.1186/s40478-020-01104-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Immunohistochemistry for GSK-3β in pinniped brains (case No. 9). The cytoplasmic uniform distribution of GSK-3β and GSK-3β-positive grains was found in the neuronal somata of aged pinnipeds (a). P-GSK-3β (Tyr216)-positive cells were found in aged pinniped brains (b). Double-labeling immunofluorescence in the cerebral cortex of aged pinnipeds revealed that p-GSK-3β (Tyr216)-positive grains were localized in AT8-positive cells (c) Reactions to AT8 are shown in red, and those to GSK-3β and p-GSK-3β (Tyr216) in green. Scale bars: 20 μm (a, b); 10 μm (c). Western blotting analysis of GSK-3β in TBS-soluble fractions from the forebrain of pinnipeds. A distinct band of GSK-3β with a molecular weight of 46 kDa was detected in pinniped brains (arrow, d). A stronger band of p-GSK-3β (Tyr216) was detected in case No. 8 and 9, in which severe hp-tau accumulation was confirmed immunohistochemically, than in other cases (arrow, e)