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. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: J Phys Chem B. 2020 Jun 4;124(28):5930–5939. doi: 10.1021/acs.jpcb.0c03028

Figure 5 – MAL and PLP may compete for cholesterol.

Figure 5 –

(A) Cholesterol depletion does not significantly affect MAL partitioning, which still enriches in raft phase upon cholesterol depletion with MβCD. (B) PLP partitioning is reduced by cholesterol depletion, becoming approximately homogeneously distributed between phases. (C) Cholesterol depletion severely reduces PLP raft affinity when co-expressed with MAL. Under this condition, PLP is excluded from rafts domains and co-enriches with the non-raft marker (F-DiD: white). (D) Quantifications of all conditions. (E) LAT partitioning is not affected by MβCD treatment. Bars represent average +/− s.e.m.; circles represent means of individual experiments (>10 vesicles each). *p<0.1, **p<0.01, ***p<0.001 by t-test. (F) PLP partitioning negatively correlates with the abundance of MAL. (i.e. relative intensity of MAL to PLP). (F) The effect is enhanced by cholesterol depletion with MβCD.