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Effects of ACTL6A on AKT signaling protein expression. (A) U251 and (B) T98G cells were transfected with shACTL6A or shCtrl and the levels of p-AKT, AKT, p-PI3K, PI3K, E-cadherin and Vimentin were detected by western blotting. The results reveal that the PI3K/AKT signaling pathway was inhibited after the knockdown of ACTL6A. ACTL6A, actin-like 6A; sh, short hairpin; Ctrl, control; p, phosphorylated. . *P<0.05, **P<0.01 and ***P<0.0001 vs. shCtrl.
