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. 2020 Dec 21;21(2):163. doi: 10.3892/etm.2020.9594

Figure 4.

Figure 4

Inflammation-associated factors stimulate the expression and activation of cell proliferation signals in ovarian cancer cells. Expression of TLR4, JNK1, p38, ERK2 and NF-κB in HEY cells treated without or (A) with LPS or LPS + anti-TLR4, (B) IL-6 or IL-6 + anti-gp130, (C) IL-8 or IL-8 + anti-CXCR2 and (D) TNF-α or TNF-α + anti-TNF-α as detected by reverse transcription-quantitative PCR analysis. GAPDH served as an internal control. Data are presented as the mean ± SEM of three independent experiments. *P<0.05 as indicated. Expression and phosphorylation levels of inflammation-associated factor receptors, molecules in the MAPK signaling pathway and NF-κB in HEY cells treated with (E) LPS or LPS + anti-TLR4, (F) IL-6 or IL-6 + anti-gp130, (G) IL-8 or IL-8 + anti-CXCR2 and (H) TNF-α or TNF-α + anti-TNF-α, as evaluated by western blotting. NF-κB, nuclear factor-κB; LPS, lipopolysaccharides; IL, interleukin; TNF, tumor necrosis factor; TLR, Toll-like receptor; MAPK, mitogen-activated protein kinase; IL-6R, IL-6 receptor; TNFR, TNF receptor; gp130, membrane glycoprotein 130.