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. 2020 Dec 22;13(1):7. doi: 10.3390/cancers13010007

Figure 1.

Figure 1

Signal transducer and activator of transcription 3 (STAT3) is phosphorylated in Malignant pleural mesothelioma (MPM) cells and its expression is required for optimal cell growth. (A) Protein expression of STAT3, phospho-STAT3 (Tyr705), and β-actin was measured by immunoblotting in established cell lines (H28, H2804, H2052, MSTO-211H (MSTO); top) and MPM primary-derived cell lines (MS1-MS4; bottom). (B) LP cells expressing active STAT3 (STAT3C) or containing a control vector (EV) were generated and growth was measured in a four-day culture (* significant differences with p < 0.05; top). Protein expression of STAT3, FLAG-tagged STAT3 and β-actin was determined by immunoblotting (bottom). (C) RNA silencing of STAT3 was performed in cell lines (MSTO-211H (MSTO), H28, LP9) and growth (n = 4) was measured using different small hairpin RNA (shRNA) expression constructs, as indicated (* significant differences with p < 0.005, left). Protein expression was measured by immunoblotting in response to STAT3 knockdown, as indicated (right). The molecular weights (in kDa) for each detected protein are indicted on the left of each immunoblot.