Table 2.

Characteristic of primer and conditions of the MS-HRM protocol.

Oxidative and Nitrosative Stresses
Gene	Starter Sequence (5′->3′)	Product Size [bp]	The Condition of the Reaction
			The Initial Activation of the Polymerase		Denaturation		Annealing		Elongation		The HRM Analysis
			Temperature [°C]	Time [min]	Temperature [°C]	Time [s]	Temperature [°C]	Time [s]	Temperature [°C]	Time [s]
Cat	F: TTTGAGATTATTGTGTTTGAAA R: TACCTACACCCAAAAAAAAATA	148	95	12	95	15	59	20	72	20	Denaturation at 95 °C for 15 s, reannealing at 60 °C for 1 min and melting from 60 to 95 °C at a ramp rate of 0.2 °C
Gpx1	F: GTTGTTTTAGGTTTTGTTGTTG R: AAAACTAAAATCCTCCAACTCT	102					65
Gpx4 (promotor 2)	F: AGGTTGGAGGTTTAGAGGTTTA R: TCCCCTAAATACAAAAATCTCT	118					59
Gpx4 (promotor 3)	F: AGGTTGGAGGTTTAGAGGTTTA R: AAAACATAACAAAATCATCTCCC	147					65
Nos1 (promotor 5)	F: GGGTTTTTAATTTTTTTATTGTG R: CAACCCTCATTAAAAAAACC	124					59
Nos1 (promotor 7)	F: GTTTGAGATTGGAATTTTTTGG R: CCAAAACATCCAAAAATACACA	124					59
Tryptophan catabolites pathway
Gene	Starter sequence (5′->3′)	Product size [bp]	The condition of the reaction
			The initial activation of the polymerase		Denaturation		Annealing		Elongation		The HRM analysis
			Temperature [°C]	Time [min]	Temperature [°C]	Time [s]	Temperature [°C]	Time [s]	Temperature [°C]	Time [s]
Tph1	F: GGGAGTTTTGTTTTGGTTTTTA R: TCCTCAACCACAAAAAATCTAA	132	95	12	95	15	55	20	72	20	Denaturation at 95 °C for 15 s, reannealing at 60 °C for 1 min and melting from 60 to 95 °C at a ramp rate of 0.2 °C
Ido1	F: TTTGAGTTTTAGTGATTTTGGG R: TTAATATCTAATCCCAATCTCTAAAAC	100					59
Tdo2 (promoter 1)	F: GATGATTTAGGTGGTTTGAGGT R: CAAAAAAAACAAAATTCATCCA	123					59
Tdo2 (promoter 2)	F: ATGATTTAGGTGGTTTGAGGTT R: ACCCAATCTACCTAACTAACAAC	187					61.4
Kmo	F: TTGGTTTAGGGAAGGAAATR: ATAAAAAACTAAACCCAAAACAC	150					55.7