Table 3.
Characteristics of antibody used in Western Blot.
| Oxidative Stress | ||||||||
| Protein | Primary Antibody | Secondary Antibody | ||||||
| Producent | The Origin of Antibodies | Dilution | Condition of Incubation [h] | Producent | The Origin of Antibodies | Dilution | Condition of Incubation [h] | |
| β-actin (a reference protein) |
Santa Cruz Biotechnology Inc, Dallas, Texas, USA | Mouse | 1:1000 | 1 h at room temperature | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Anti-mouse | 1:6000 | 1 h at room temperature |
| catalase | Santa Cruz Biotechnology Inc, Dallas, Texas, USA | Mouse | 1:1000 | overnight at 4 °C | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Anti-mouse | 1:6000 | 1 h at room temperature |
| glutathione peroxidase 4 | Abcam, Cambridge, United Kingdom, | Rabbit | 1:6000 | overnight at 4 °C | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Anti-rabbit | 1:6000 | 1 h at room temperature |
| superoxide dismutase 1 | Santa Cruz Biotechnology Inc, Dallas, Texas, USA | Mouse | 1:6000 | 2 h at room temperature | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Anti-mouse | 1:6000 | 1 h at room temperature |
| Tryptophan catabolites pathway | ||||||||
| Protein | Primary antibody | Secondary antibody | ||||||
| Producent | The origin of antibodies | Dilution | Condition of incubation [h] | Producent | The origin of antibodies | Dilution | Condition of incubation [h] | |
| Tryptophan hydroxylase 1 | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Rabbit | 1:1000 | overnight at 4 °C | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Anti-rabbit | 1:6000 | 1 h at room temperature |
| Tryptophan hydroxylase 2 | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Rabbit | 1:6000 | overnight at 4 °C | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Anti-rabbit | 1:6000 | 1 h at room temperature |
| Indoleamine 2,3-dioxygenase | Santa Cruz Biotechnology Inc, Dallas, Texas, USA | Mouse | 1:1000 | overnight at 4 °C | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Anti-rabbit | 1:6000 | 1 h at room temperature |
| Kynurenine aminotransferases | Santa Cruz Biotechnology Inc, Dallas, Texas, USA | Mouse | 1:1000 | overnight at 4 °C | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Anti-rabbit | 1:6000 | 1 h at room temperature |
| Kynureninase | Santa Cruz Biotechnology Inc, Dallas, Texas, USA | Mouse | 1:1000 | overnight at 4 °C | Cell Signalling Technologies Inc., Danvers, Massachusetts, USA | Anti-rabbit | 1:6000 | 1 h at room temperature |