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. 2020 Dec 22;13(1):4. doi: 10.3390/cancers13010004

Figure 6.

Figure 6

In vitro motility and invasion of OVCAR-3 and OV-90 cells after overexpressing piRNA pathway genes and its mutants (n = 21–24 per cell line per transfection). (a,b) Motility: PIWIL1 and PL2L60 (PIWIL2 mutant) overexpression significantly decreased OVCAR-3 motility while PIWIL2 overexpression significantly decreased motility in both OVCAR-3 and OV-90. OVCAR-3 cells overexpressing P1∆17 (PIWIL1 mutant) had increased motility compared to empty vector and was significantly more motile than PIWIL1 overexpression. (c,d) Invasion: overexpression of PIWIL1, PIWIL2, PL2L60 and MAEL significantly decreased OVCAR-3 invasion while PIWIL2 overexpression significantly decreased OV-90 invasion. OV-90 cells overexpressing PL2L60 had significantly increased invasion compared to PIWIL2 overexpression. All fold changes were calculated relative to empty vector. Three biological replicates were carried out per cell line per transfection group. Bar represents median values plotted for all the datasets. Statistical tests Mann−Whitney U (non-normal distribution) or unpaired t-test (normal distribution) were used accordingly. * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001.