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Considerations for soluble Klotho measurements in human samples
| Sample Processing | Klotho Assay |
|---|---|
| Sample type and quality | Methods of measurement (ELISA versus other) |
| Sample vintage (from sample collection to processing) | Reagents |
| Sample vintage (from sample storage to measurement) | Specificity of antibodies |
| Methods of collection | Additives (e.g., protease inhibitors) |
| Methods of storage | Interassay variability |
| Freeze-thaw cycles | Intra-assay variability |
| High-throughput capacity |
