Fig. 8.

Effects of polymyxin B (Pmx B) and heating on dust extracellular vesicle (EV) induction of inflammatory mediator protein levels in Beas2B cells. A–E: to determine the effects of Pmx B, cells were first incubated with Pmx B (10 μg/mL) for 30 min and then treated with EVs (0.25 μg protein/mL) for 5 h. Control cells (C) were left untreated. The levels of proIL-1β and ICAM-1 in cell lysates and the levels of IL-8 and IL-6 in cell medium were determined. A representative Western blot with noncontiguous lanes demarcated by a broken line is shown. Data shown are means ± SE (n = 6). **P < 0.01 and ****P < 0.0001 according to one-way ANOVA using Tukey’s multiple-comparison test. F–J: cells were untreated (C) or treated with medium containing EVs (0.5 μg protein/mL), EVs (0.5 μg protein/mL) heated at 95°C for 10 min (EVs 95°C), dust extract (0.25%) (DE), or dust extract (0.25%) heated at 95°C for 10 min (DE 95°C) for 5 h, and the levels proIL-1β and ICAM-1 in cell lysates and the levels of IL-6 and IL-8 proteins in cell medium were determined. Data shown are means ± SE (n = 4). A representative Western blot is shown. *P < 0.05 and **P < 0.01 according to one-way ANOVA using Tukey’s multiple-comparison test.