Fig. 7.

Pulmonary hypertension (PH) indices at PND14 in C57BL/6J wild-type mice exposed to LPS and hyperoxia during the saccular phase of lung development. High-resolution echocardiography was performed on postnatal day (PND) 14 in C57BL/6J wild-type mice exposed to 21% O₂ (normoxia) or 70% O₂ (hyperoxia) during PNDs 1–5 and injected intraperitoneally with 6 mg/kg of LPS (L6) or the vehicle (PBS) on PNDs 3–5. Representative PWD echocardiography recordings of pulmonary artery blood flow obtained from mice treated with PBS (A and C) or L6 (B and D) and exposed to normoxia (A and B) or hyperoxia (C and D). PAT (E), PAT/ET ratio (F), RVSP (G), and heart rate (H) were estimated from the PWD Echo pulmonary artery blood flow recordings. Values are expressed as means ± SD (n = 5 or 6 mice/group). Significant differences between PBS- and LPS-treated mice under hyperoxic conditions are indicated by †P < 0.05. Significant differences between treatment-matched mice under normoxic and hyperoxic conditions are indicated by §P < 0.05.