Fig. 2.

15-Hydoxyprostaglandin dehydrogenase inhibition ameliorates renal tubular cell death in mice with ischemic acute kidney injury. Before and after renal ischemia-reperfusion injury (IRI), mice were injected intraperitoneally three times with vehicle, SW033291 (5 mg/kg), or indomethacin (5 mg/kg). Assessments were performed at 24 h after renal IRI. A: representative gross appearance of the right (Rt) and left (Lt) kidneys of mice injected with vehicle (IRI-vehicle), indomethacin (IRI-indomethacin), or SW033291 (IRI-SW033291) before and after renal IRI. Vascular congestion in the renal medulla is indicated by white arrows. B: representative image of tubular injury in the outer zone of the renal medulla (hematoxylin and eosin staining). Scale bars in the enlarged images = 50 μm; scale bars in insets = 500 μm. C: statistical analysis of tubular injury scores (n = 20 per group). D: representative images of apoptosis in the outer zone of the renal medulla (TUNEL staining). Scale bar in the enlarged images = 25 μm; scale bars in insets = 500 μm.;. E: statistical analysis of apoptosis (n = 20 per group). Data are presented as means ± SE. Analysis was performed using Student’s t test.