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. 2020 Oct 5;319(6):F1015–F1026. doi: 10.1152/ajprenal.00405.2020

Fig. 6.

Fig. 6.

Effect of TMP195 on the expression of BAX, phosphorylated (p-)Bcl-2, bone morphogenetic protein-7 (BMP-7), histone deacetylase (HDAC)4, and acetyl histone H3K14 in cultured murine renal epithelial cells exposed to lipopolysaccharide (LPS). Serum-starved murine proximal tubular cells were exposed to LPS for 24 h in the absence or presence of TMP195 and then harvested. Cell lysates were subjected to immunoblot analysis with antibodies to cleaved caspase-3, p-Bcl-1, BAX, BMP-7, β-actin, α-tubulin, or GAPDH (A); HDAC4 and acetyl-H3K14 (F); as well as HDAC4, acetyl-histone H3K14, and α-tubulin. B: expression levels of cleaved caspase-3 were quantified by densitometry and normalized with β-actin. C: expression levels of p-Bcl2 were quantified by densitometry and normalized with α-tubulin. D and E: expression levels of BAX (D) and BMP-7 (E) were quantified by densitometry and normalized with GAPDH. G and H: expression levels of HDAC4 (G) and acetyl histone H3K14 (H) were quantified by densitometry and normalized with α-tubulin. **P < 0.01; ***P < 0.001.