Fig. 7.

Mutation in ERα tended to increase Timp1 and significantly reduced Klk10 and Jun mRNA expression in the RV in response to PAB in females. Effects of ERα mutation and PAB on female RV gene expression of Col1a1 (A), Col3a1 (B), Mmp9 (C), Timp1 (D), kallikrein-related peptidase 10 (Klk10) (E), and Jun (F) are shown. Data are presented as means ± SE. *P < 0.05, PAB vs. Sham of same sex, and †P < 0.05, ERαMut vs. WT of same sex and stress (2-way ANOVA with Bonferroni test for multiple comparison). n = 3–4 for each group. ERα, estrogen receptor α; Klk10, kallikrein-related peptidase 10; PAB, pulmonary artery banding; RV, right ventricular; WT, wild type.