Table 3.

Nomenclature, gene information, and mRNA primer characteristics

Common Name	Gene Name	Accession No.	Sequence (5′→3′)	Amplicon Size, bp	mRNA Region, bp	Annealing Temp, °C
Toll-like receptors
TLR1	TLR1	NM_003263.3	TCCCGGAGGCAATGCTGCTGT ACAGATTCCTTTTGTAGGGGTGCCCA	145	70–214	61
TLR2	TLR2	NM_003264.4a	GCAGGATCCAAAGGAGACCT CCAGTGCTTCAACCCACAAC	129	125–253	61
TLR3	TLR3	NM_003265.2	GCAAAAGATTCAAGGTACATCATGC CCTCTTCGCAAACAGAGTGC	127	2581–2707	61
TLR4	TLR4	NM_003266.3b	CCGTTTTATCACGGAGGTGGT GGGAGGTTGTCGGGGATTTT	81	496–576	61
TLR5	TLR5	NM_003268.5	ACGAGGATCATGGGAGACCA ATTCGGCCATCAAAGGAGCA	98	633–730	61
TLR6	TLR6	NM_006068.4	GCAGGGGACAATCCATTCCA AGAATCAGGCCAGCCCTCTA	99	1697–1795	61
TLR7	TLR7	NM_016562.3	GGCCCATCTCAAGCTGATCT GTCCACATTGGAAACACCATTT	106	54–159	61
TLR8	TLR8	NM_016610.3c	ACTTGACCCAACTTCGATACCT CAGCACCTTCAGATGAGGCA	95	1078–1172	61
TLR9	TLR9	NM_017442.3	TACCTTGCCTGCCTTCCTAC TGACATTGCCACGGGGTG	116	715–830	60
TLR10	TLR10	NM_030956.3d	TATACAGGGTTTTGAGCTCATCTT GCATCACCCTCTGCTGTCAT	131	570–700	61
Adaptors
Myd88	MYD88	NM_001172569.1e	TCTCCAGGTGCCCATCAGAA AAGGCGAGTCCAGAACCAAG	138	637–774	61
TRIF*	TICAM1	NM_182919.3	CATCCTCGGCTTTGCCCTC GGGGACTGGCTGATTTCCAA	139	768–906	61
Signaling components
IKKβ†	IKBKB	NM_001556.2f	ATGTCATCCGATGGCACAATCAGG TGGGTCAGCCTTCTCATGATCTGG	127	260–386	60
IκBα‡	NFKBIA	NM_020529.2	CCAACTACAATGGCCACACGTGTCTACA GAGCATTGACATCAGCACCCAAGG	99	646–744	60

For primer sequences, top sequence reflects the forward primer and bottom sequence reflects the reverse primer. Other aliases:

^*Toll-like receptor adaptor molecule 1;

^†Inhibitor of nuclear factor kappa B kinase subunit beta;

^‡NF-κB inhibitor alpha.

^aPrimers detect variant 2 (NM_001318789.1), variant 3 (NM_003264.4), variant 4 (NM_001318790.1), and variant 6 (NM_001318793.1).

^bPrimers detect variant 1 isoform a (NM_138554.4) and variant 3 isoform c (NM_003266.3).

^cPrimers detect variant 1 isoform 1 isoform 1 (NM_016610.3) and variant 2 isoform 2 (NM_138636.5).

^dPrimers detect all variants encoding isoform a: variant 1 (NM_030956.3), variant 2 (NM_001017388.2) variant 3 (NM_001195106.1), and variant 4 (NM_001195107.1).

^ePrimers detect all variants: variant 1 isoform 1 (NM_001172567.1), variant 2 isoform 2 (NM_002468.4), variant 3 isoform 3 (NM_001172568.1), variant 4 isoform 4 (NM_001172569.1), and variant 5 isoform 5 (NM_001172566.1).

^fPrimers detect variant 1 isoform 1 (NM_001556.2), variant 2 isoform 2 (NM_001190720.2), and variant 7 isoform 5 (NM_001242778.1).