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. 2020 Dec 22;22(1):29. doi: 10.3390/ijms22010029

Figure 3.

Figure 3

C18GEM induced an increase in regulated cell death compared to GEM in both cell lines grown on 2D or three-dimensional (3D) cultures. Annexin V fluorescence intensity in Panc1 parental (P) cells and CSCs treated with 50 μM of GEM or C18GEM for 48 h in 2D conditions (A) or 72 h in Matrigel- and collagen I-rich ECM, as described in Material and Methods (B). Ethidium homodimer fluorescence intensity of P cells and CSCs treated with 50 μM of GEM or C18GEM for 72 h in Matrigel- and collagen I-rich ECM (C). The values are reported as fold change relative to untreated cells and are the means (±SE) of at least three independent biological replicates. Statistical legend: p < 0.05 (*), p < 0.01 (**), or p < 0.001 (***) GEM or C18GEM versus CTRL; p < 0.05 (§), p < 0.01 (§§), or p < 0.001 (§§§) C18GEM versus GEM.