Figure 3.

Two-dimensional ¹⁵N-detected CRINEPT spectra of mAb in formulated storage condition. (A) The pulse sequence of the ¹⁵N-detected CRINEPT experiment. Narrow bars indicate π/2 hard pulses. The phase cycles employed were φ1 = (x−x), φ2 = y, φ3 = (x−x−x−x), and φrec = (x−x−x−x). Phase-sensitive acquisition in the indirect ¹H dimension is achieved by incrementing the phase φ1 in a STATES-TPPI manner [71]. (B) Examples of formulation condition for subcutaneous injection (SC; left), and intravenous injection (IV; right) (C) The ¹⁵N-detected CRINEPT spectra of the mAb in the SC (left) and IV (right) formulations. These spectra were acquired at 4 °C. The figure is reproduced from [67].