Figure 6.

Superresolution imaging of a breast cancer (SkBr3) cell during DSB repair after exposure to 1 Gy X-rays. From such images, characteristic molecular arrangements during repair are elucidated. (A) Overview image acquired by widefield microscopy. The blue square (2 µm × 2 µm) encloses a typical γH2AX focus. (B) Superresolution SMLM image of heterochromatin (green) and γH2AX foci (red), with the γH2AX overview image in the background indicating the reduced z-slice depth in the SMLM image reconstructed from the label point coordinates. (C) Magnification of the marked region (2 µm × 2 µm) in the SMLM image with Gaussian blur but without the background image; the two color channels are separated in the upper and lower images. (D) The same image as (C) but with maximum precision of label points (each point corresponds to a single fluorescent molecule of the indicated antibody). Maximum precision means the highest image resolution that can be obtained from the SMLM data set. (Note: In all images, the blue squares enclose an area of 2 µm × 2 µm and can be used as scale bars.).