Skip to main content
. 2020 Dec 23;13(1):21. doi: 10.3390/cancers13010021

Figure 4.

Figure 4

Homologous recombination deficient cells are sensitive to 5-azaC and HPV-positive head and neck cancer cells are deficient in a homologous recombination repair. (A) Clonogenic survival of Chinese hamster ovary cells lacking BRCA2 (VC-8) and cells with restored BRCA2 (VC-8 B2) after treatment with increasing doses of 5-azaC. Standard deviation calculated from 2 independent experiments. Student’s t test was performed to test significance. (B) Representative immunofluorescent images (left) and quantification (right) of ɣH2AX and Rad51 foci formation in HPV-negative (top) and HPV-positive (bottom) HNSCC cells after HU treatment. Scale bars, 50 μm. Values indicate the mean ± SD with n ≥ 50 cells in 2 biological replicates. Student’s t test was performed to test significance. * indicates p-value < 0.05; n/s (not significant) indicates p-value > 0.05. (C) Homologous recombination reporter results in HPV-positive (UMCSS47 and SCC090) and HPV-negative (UNC7 and SCC61) HNSCC cells stably expressing pDRGFP and transfected or not with I-SCE1. Values indicate the mean ± SD from 2 biological replicates. Student’s t test was performed to test significance. * indicates p-value < 0.05; n/s (not significant) indicates p-value > 0.05.