Table 1.
Solution properties of DDX17135–555, IGR, and 5’ NCR.
| Sample | DDX17135_555 | IGR | 5’ NCR |
|---|---|---|---|
| Mw (kDa, sequence) | 48.45 | 23.82 | 24.70 |
| Sedimentation coefficient, S (10−13 s) ∇ | 3.16 | 4.07 | 4.18 |
| Diffusion coefficient D (10−7 cm2/s) ∇ | 5.22 | 7.62 | 6.58 |
| Rh (Å) ∇ | 41.06 | 28.11 | 32.64 |
| I(0) # | 0.003 ± 2.5 × 10−5 | 0.026 ± 4.4 × 10−5 | 0.022 ± 2.6 × 10−4 |
| q.Rg range # | 0.39–1.30 | 0.26–1.29 | 0.40–1.29 |
| Rg (Å) # | 24.78 ± 0.36 | 36.42 ± 0.10 | 50.44 ± 0.88 |
| I(0) ∆ | 0.003 ± 2.3 × 10−5 | 0.026 ± 4.3 × 10−5 | 0.019 ± 1.7 × 10−4 |
| Rg (Å) ∆ | 25.46 ± 0.27 | 38.00 ± 0.08 | 46.66 ± 0.34 |
| Dmax (Å)∆ | 79.21 | 122 | 148 |
| Χ2 * | ~1.00 | ~1.10 | ~1.30 |
| NSD * | 0.52 ± 0.02 | 0.73 ± 0.02 | 0.58 ± 0.01 |
The Mw values were calculated using nucleotide sequences. ∇—determined using SV-AUC analysis and UltraScan-III package [28]. Sedimentation coefficients obtained following genetic algorithm–Monte Carlo analysis. #—obtained from Guinier analysis [21]. ∆—determined using P(r) analysis using the GNOM program [24]. *—values derived from DAMMIN [26] and DAMAVER [27] analysis. Rh – hydrodyhamic radius; Rg – radius of gyration; Dmax – maximum particle dimension; NSD: normalized spatial discrepancy.